Posts

“Sequence Duplication Levels” module still fails after pre-processing Illumina data

Image
2 $begingroup$ I want to ask about why the sequence duplication levels are high after I trimmed by using Trimmomatic? I am using the following Trimmomatic operations: HEADCROP = 19 TRAILING = 20 MINLEN = 66 . How can i solve this problem? Thank You. illumina data-preprocessing trimming fastqc share | improve this question edited Jan 10 at 16:14 Daniel Standage 2,150 3 27 asked Jan 10 at 14:35 yy97 yy97 13 2 ...